Host:

E.coli

Tag:

His-tag

AA Sequence:

CCTATTAACCCGAAAGGCCTGGAACTGGATCCGGAAGGTAGTCTGCATCATCAGCAGAAACGTGAAGCACCGAGTCGCAGTAGTGCCAGTAGCGGCCCGCAGATTCTGAAATGCCCGGAAGCCGAATGCTTCCGCCTGCGCTGTGAACTGGGCCCGCTGCATCAGCAGGAAAGCCAGAGCCTGCAGCTGCACTTCCGTGTGTGGGCAAAAACCTTCCTGCAGCGTGAACATCAGCCGTTCAGCCTGCAGTGCGAAGCAGTGTATAAAGCACTGAAAATGCCGTATCGTATTCTGCCGCGCCAGCTGCCGCAGAAAGAACGCCAGGTTGCAACCGCAGTGCAGTGGACCAAAGCAGAAGGCAGCTAT

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~16kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival. The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities. Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling). Integrin α5/β1 is involved in multiple biological processes including embryonic development, angiogenesis and tumor metastasis. By interaction with its fibronectin ligand, α5/β1 transduces signals that regulate cell adhesion, migration, matrix assembly and cytoskeletal organization.

Note:

For research use only, not for use in diagnostic procedure.