Bioworld Technology

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RecombinantTNF-α,Human(P.pastoris-expressed) BK0347

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Specifications

size	10μg / 50μg / 1mg

Source:

P. pastoris

Molecular Weight:

17.4kDa, observed by reducing SDS-PAGE.

Purity:

> 95% by SDS-PAGE analysis.

Biological activity:

ED50<0.08ng/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of >1.25 x 10ˆ7units/mg.

AA Sequence:

VRSSSR TPSDKPVAHV VANPQAEGQL QWLNRRANAL LANGVELRDN QLVVPSEGLY LIYSQVLFKG QGCPSTHVLL THTISRIAVS YQTKVNLLSA IKSPCQRETP EGAEAKPWYE PIYLGGVFQL EKGDRLSAEI NRPDYLDFAE SGQVYFGIIA L

Endotoxin:

<1.0 EU/μg, determined by LAL method.

Formulation:

Lyophilized after extensive dialysis against PBS.

Background:

Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.Recombinant human Tumor Necrosis Factor-Alpha (rhTNF-alpha) produced in Pichia pastoris is a glycosylated polypeptide chain of 157 amino acids. A fully biologically active molecule, rhTNF-alpha has a molecular mass of 17.4kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.

Usage:

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution:

Reconstituted in ddH2O at 100 μg/ml.

Storage:

Lyophilized recombinant human Tumor Necrosis Factor-Alpha (rhTNF-alpha) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhTNF-alpha should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.