Source:

Escherichia coli.

Molecular Weight:

26.2kDa, observed by non-reducing SDS-PAGE.

Purity:

> 95% by SDS-PAGE and HPLC analyses.

Biological activity:

ED50 < 2.0 ng/ml, measured by a cell proliferation assay using TF-1 Cells, corresponding to a specific activity of > 5.0× 10ˆ5 units/mg.

AA Sequence:

MEIPMSTVV KETLTQLSAH RALLTSNETM RLPVPTHKNH QLCIGEIFQG LDILKNQTVR GGTVEMLFQN LSLIKKYIDR QKEKCGEERR RTRQFLDYLQ EFLGVMSTEW AMEG

Endotoxin:

< 0.2 EU/μg, determined by LAL method.

Formulation:

Lyophilized after extensive dialysis against PBS.

Background:

Interleukin-5 (rmIL-5),produced by mast cells, T cells and eosinophils, is responsible for the activities attributed to eosinophil differentiating factor, B cell growth factor II and T cell-replacing factor (TRF). It can increase production and mobilization of eosinophils and CD34+ progenitors from the bone marrow. IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release.Recombinant mouse Interleukin-5 (rmIL-5) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 113 amino acids each. A fully biologically active molecule, rmIL-5 has a molecular mass of 26.2kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Usage:

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution:

Reconstituted in ddH2O at 100 μg/ml.

Storage:

Lyophilized recombinant mouse Interleukin-5 (rmIL-5) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIL-5 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.