Source:

Escherichia coli.

Molecular Weight:

15.2 kDa, observed by non-reducing SDS-PAGE.

Purity:

> 95% as analyzed by SDS-PAGE and HPLC.

Biological activity:

ED50 < 0.5 ng/mL, measured by a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 2 × 10ˆ7 units/mg.

AA Sequence:

MAPMTQTTP LKTSWVNCSN MIDEIITHLK QPPLPLLDFN NLNGEDQDIL MENNLRRPNL EAFNRAVKSL QNASAIESIL KNLLPCLPLA TAAPTRHPIH IKDGDWNEFR RKLTFYLKTL ENAQAQQTTL SLAIF

Endotoxin:

< 0.2 EU/μg, determined by LAL method.

Formulation:

Lyophilized after extensive dialysis against PBS.

Background:

Interleukin-3 (IL-3) is a pleiotropic cytokine belonging to the interleukin family. IL-3 shares similarities with Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) and IL-5: they all have a four-helix bundle structure, are located on the same chromosomes in both human and mouse, are produced by activated T cells, and share receptors. The IL-3/IL-5/GM-CSF receptor family members are all heterodimeric, composed of a receptor-specific α chain and a common β chain. IL-3 is also called multi-colony stimulating factor since it stimulates the development and colony formation of multiple lineages of hematopoietic cells by activating intracellular pathways such as Ras-Raf-ERK and JAK/STAT. IL-3 inhibits apoptosis and promotes cell survival by targeting the anti-apoptotic bcl-2 gene family. Recombinant human Interleukin-3 (rhIL-3) produced in E.coli is a single non-glycosylated polypeptide chain containing of 134 amino acids. A fully biologically active molecule, rhIL-3 has a molecular mass of 15.2 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Usage:

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution:

Reconstituted in ddH2O at 100 μg/mL.

Storage:

Lyophilized recombinant human Interleukin-3 (rhIL-3) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIL-3 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.