Source:

Escherichia coli.

Molecular Weight:

17.7 kDa, observed by reducing SDS-PAGE.

Purity:

> 95% as analyzed by SDS-PAGE.

Biological activity:

ED50 < 1ng/ml, measured by the dose-dependent stimulation of mouse D10S cells, corresponding to a specific activity of 1.0× 10ˆ6 IU/mg.

AA Sequence:

MANVQSMEC KLQDKDHKSL VLAGPHMLKA LHLLTGDLKR EVVFCMSFVQ GDDSNNKIPV TLGIKGKNLY LSCVMKDNTP TLQLEDIDPK RYPKRDMEKR FVFYKTEIKN RVEFESALYP NWYISTSQAE QKPVFLGNSK GRQDITDFTM EVLSP

Endotoxin:

< 0.2 EU/μg, determined by LAL method.

Formulation:

Lyophilized after extensive dialysis against PBS.

Background:

Interleukin-1 beta (rpIL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. IL-1α and IL-1β are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. While IL-1α and IL-1β are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1β stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity.Recombinant Porcine IL-1β produced in E.coli is a single non-glycosylated polypeptide chain containing 154 amino acids. A fully biologically active molecule, rpIL-1β has a molecular mass of 17.7 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Usage:

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution:

Reconstituted in ddH2O or PBS at 100 μg/ml.

Storage:

Lyophilized recombinant Porcine IL-1β, remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Porcine IL-1β should be stable up to 1 week at 4°C or up to 3 months at -20°C.