Source:

Escherichia coli.

Molecular Weight:

17.5kDa, observed by reducing SDS-PAGE.

Purity:

> 95% by SDS-PAGE and HPLC analyses.

Biological activity:

ED50<2.0 pg/ml, measured by the dose-dependent stimulation of mouse D10S cells, corresponding to a specific activity of> 5.0× 10ˆ8units/mg.

AA Sequence:

MVPIRQLHYR LRDEQQKSLV LSDPYELKAL HLNGQNINQQ VIFSMSFVQG EPSNDKIPVA LGLKGKNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKSK VEFESAEFPN WYISTSQAEH KPVFLGNNSG QDIIDFTMES VSS

Endotoxin:

<0.2 EU/μg, determined by LAL method.

Formulation:

Lyophilized after extensive dialysis against PBS.

Background:

Interleukin-1 Beta (IL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.Recombinant mouse Interleukin-1 Beta(rmIL-1β) produced in E. coli is a single non-glycosylated polypeptide chain containing 153 amino acids. A fully biologically active molecule, rmIL-1β is obtained by proprietary chromatographic techniques at GenScript, with an apparent molecular weight of 17.5kDa analyzed by reducing SDS-PAGE.

Usage:

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution:

Reconstituted in ddH2O or PBS at 100 μg/ml.

Storage:

Lyophilized recombinant mouseInterleukin-1 Beta(rmIL-1β) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIL-1β should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.