Bioworld Technology

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RecombinantFGF-8f,Human BK0048

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Specifications

size	10μg / 50μg / 1mg

Source:

Escherichia coli.

Molecular Weight:

25.5 kDa, observed by reducing SDS-PAGE.

Purity:

> 95% by SDS-PAGE analysis.

Biological activity:

ED50 < 50ng/mL, measured by a cell proliferation assayusing 3T3 cellsin the presence of 10 μg/ml of heparin, corresponding to a specific activity of > 2× 10ˆ4 units/mg.

AA Sequence:

MQEGPGRGP ALGRELASLF RAGREPQGVS QQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS GKHVQVLANK RINAMAEDGD PFAKLIVETD TFGSRVRVRG AETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG HHTTEQSLRF EFLNYPPFTR SLRGSQRTWA PEPR

Endotoxin:

< 0.2 EU/μg, determined by LAL method.

Formulation:

Lyophilized after extensive dialysis against PBS.

Background:

Fibroblast Growth Factor 8f (FGF-8f) is a cytokine belonging to the heparin-binding FGF family, which has at least 23 members. FGF-8 has 8 different isoforms, named FGF-8a through FGF-8h. Different FGF-8 isoforms have different receptor affinities, and thus participate in different signaling cascade pathways. FGF-8 has widespread expression during embryonic development, promoting gastrulation, somitogenesis, morphogenesis, and limb formation. FGF-8 also has oncogenic potential. While in normal cells FGF-8 is expressed at very low levels, in breast, prostate and ovarian cancer FGF-8 is highly expressed.FGF-8 promotes tumor angiogenesis by increasing neovascularization, and inducing osteoblastic differentiation. Recombinant human Fibroblast Growth Factor 8f (rhFGF-8f) produced in E.coli is a single non-glycosylated polypeptide chain containing 223 amino acids. A fully biologically active molecule, rhFGF-8f has a molecular mass of 25.5 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Usage:

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution:

Reconstituted in ddH2O at 100 μg/mL.

Storage:

Lyophilized recombinant human Fibroblast Growth Factor 8f(rhFGF-8f) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-8f remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.