RecombinantFGF-18,Human BK0037
Specifications
| 10μg / 50μg / 1mg |
Source:
Escherichia coli.
Molecular Weight:
20.3 kDa, observed by reducing SDS-PAGE.
Purity:
> 95% by SDS-PAGE and HPLC analyses.
Biological activity:
ED50 < 10 ng/ml, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 1.0× 10ˆ5units/mg.
AA Sequence:
MAEENVDFR IHVENQTRAR DDVSRKQLRL YQLYSRTSGK HIQVLGRRIS ARGEDGDKYA QLLVETDTFG SQVRIKGKET EFYLCMNRKG KLVGKPDGTS KECVFIEKVL ENNYTALMSA KYSGWYVGFT KKGRPRKGPK TRENQQDVHF MKRYPKGQPE LQKPFKYTTV TKRSR
Endotoxin:
< 0.2 EU/μg, determined by LAL method.
Formulation:
Lyophilized after extensive dialysis against PBS.
Background:
Fibroblast Growth Factor-18 (FGF-18) is a heparin-binding growth factor that is a member of the FGF family. FGF-18 signals through FGFR 1c, 2c, 3c, and 4. FGF-18 plays an important role in the regulation of cell proliferation, cell differentiation and cell migration. FGF-18 is required for normal ossification and bone development. It can also stimulate hepatic and intestinal proliferation.Recombinant human Fibroblast Growth Factor-18 (rhFGF-18) produced in E.coli is a single non-glycosylated polypeptide chain containing 174 amino acids. A fully biologically active molecule, rhFGF-18 has a molecular mass of 20.3 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Usage:
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Reconstitution:
Reconstituted in ddH2O at 100 μg/ml.
Storage:
Lyophilized recombinant human Fibroblast Growth Factor-18 (rhFGF-18) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-18 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
