Bioworld Technology

Bioworld Technology is manufacturer of over 15,000 highly purified Monoclonal and Polyclonal Antibodies, Peptides, Proteins, and other related Research Products. Their products are used in all biological fields. An important focus in research taking place today is in work investigating Signal transduction pathways, Cardiac markers, Neuroscience as well as Stem cell research. Bioworld Technology is committed to providing customers with innovative research tools and to helping scientists determine the mechanisms of cell function and disease.
Bioworld Technology is one of the leading phospho-antibody manufacturers. They have produced over 500 phospho-antibodies for AKT, AMPK, GSK, STAT pathways. The peptides corresponding to each phospho-antibody have also been listed to help scientists.
All of their high quality antibodies, peptides and kits were tested in their own facility with original published pictures included. They have a commitment to customer service and offer 100% quality satisfaction. If our products do not match the results listed on the data sheet, we will help you to troubleshoot or refund for the full credit.
Bioworld Technology continues to manufacture product lines to the highest standards; and all of their scientists are dedicated to the mystery of the world of science.

RecombinantEGF,Rat BK0028

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Specifications

size	20μg / 100μg / 1mg

Source:

Escherichia coli.

Molecular Weight:

6.3 kDa, observed by reducing SDS-PAGE.

Purity:

> 95% by SDS-PAGE analysis.

Biological activity:

ED50 < 0.08 ng/mL, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 1.25× 10ˆ7 units/mg.

AA Sequence:

MNSNTGCPP SYDGYCLNGG VCMYVESVDR YVCNCVIGYI GERCQHRDLR WWKLR

Endotoxin:

< 0.2 EU/μg, determined by LAL method.

Formulation:

Lyophilized after extensive dialysis against PBS.

Background:

Epidermal Growth Factor (EGF) is a cytokine with 53 amino acids, originally found in mouse submaxillary gland. EGF binds to EGF receptors, ErbB1 and B4, and causes them to be dimerized and phosphorylated. The dimerized and phosphorylated EGFR can bind to several intracellular targets, such as phospholipase Cγ and Ras-GTPase-acting protein, and achieve a series of cascade reactions. EGF is involved in the regulation of cell proliferation and differentiation, and is up-regulated during wound healing, accelerating reepitheliazation and increasing tensile strength. It also stimulates neurite outgrowth and increases the uptake of dopamine in the central nervous system. On the other hand, EGF is up-regulated in the glioma cancer, and related to the length of survivals of the patients.Recombinant rat Epidermal Growth Factor (rrEGF) produced in E.coli is a single non-glycosylated polypeptide chain containing 54 amino acids. A fully biologically active molecule, rrEGF has a molecular mass of 6.3 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Usage:

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution:

Reconstituted in ddH2O at 100 μg/mL.

Storage:

Lyophilized recombinant rat Epidermal Growth Factor (rrEGF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrEGF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.