Source:

Escherichia coli.

Molecular Weight:

24.7 kDa, observed by reducing SDS-PAGE.

Purity:

> 95% as analyzed by SDS-PAGE and HPLC.

Biological activity:

ED50 < 5 µg/mL, measured by a cell growth inhibitory assay using M1 cells, corresponding to a specific activity of > 2× 10ˆ2 units/mg.

AA Sequence:

METTTQGPG VLLPLPKGAC TGWMAGIPGH PGHNGAPGRD GRDGTPGEKG EKGDPGLIGP KGDIGETGVP GAEGPRGFPG IQGRKGEPGE GAYVYRSAFS VGLETYVTIP NMPIRFTKIF YNQQNHYDGS TGKFHCNIPG LYYFAYHITV YMKDVKVSLF KKDKAMLFTY DQYQENNVDQ ASGSVLLHLE VGDQVWLQVY GEGERNGLYA DNDNDSTFTG FLLYHDTN

Endotoxin:

< 0.2 EU/μg, determined by LAL method.

Formulation:

Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH8.0.

Background:

Adiponectin is a hormone mainly produced by adipocytes. Adiponectin forms a homotrimer and exists as higher order multimers in vivo. The receptors of Adiponectin are seven-transmembrane G protein coupled receptors: Receptor 1 is expressed in skeletal muscle and Receptor 2 in liver. Adiponectin receives a lot of attention because of its anti-diabetic, anti-atherosclerotic, and anti-inflammatory properties. Adiponectin increases the expression of molecules involved in fatty acid transport, combustion of fatty acid, and energy dissipation, and increases insulin sensitivity of the body. Decreased levels of Adiponectin are associated with hypertension, cardiovascular diseases, and metabolic syndromes. Therefore, Adiponectin has promising potential as a pharmacological agent. Recombinant human Adiponectin (rhAdiponectin) produced in E.coli is a single non-glycosylated polypeptide chain containing 227 amino acids. A fully biologically active molecule, rhAdiponectin has a molecular mass of 24.7 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Usage:

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution:

Reconstituted in ddH2O at 100 μg/mL.

Storage:

Lyophilized recombinant human Adiponectin (rhAdiponectin) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhAdiponectin remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.